IL-1a, human, recombinant
Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.
PRODUCED TO ORDER
ISOkine™ recombinant human IL-1a is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
Sterile filtered through a 0.2 µm filter. Lyophilized from PBS, pH 7.2
Greater than 95% by SDS-PAGE gel analysis.
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
The bioactivity is determined by the cell proliferation assay for IL-1a for dose-dependent cell proliferation of the interleukin-1 (IL-1) sensitive murine T-helper cell line D10S, a stable line which proliferates to subfemtomolar concentrations of IL-1 in the absence of mitogens. The effect of serial dilutions of IL-1a on the proliferation of D10S cells is monitored at OD490 nm. The ED50 for this ISOkineTM recombinant human IL-1a lies in the range of 2.2-3.3 pg/ml corresponding to 4x 10E8 U/mg.
Endotoxin level is less than 0.005ng per µg of ISOkine™ product (0.05EU/µg) as measured by turbidimetric kinetic assay.* * Ref. Associates of Cape Cod Industries, Deacon Park, Knowsley, Liverpool, UK
Purified ISOkine™ product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.** ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland
Recombinant human IL-1a contains 159 amino acids and a 16 a.a. Histidine-based tag for a total length of 175 a.a. and has a predicted molecular mass of 20.2 kDa. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 24 and 25 kDa in SDS-PAGE