IL-5, human, recombinant
IL-5 (T cell replacing factor (TRF), eosinophil differentiation factor (EDF)) is a hematopoietic growth factor that stimulates the proliferation, differentiation and activation of eosinophils. It is produced by mast cells, T cells and eosinophils and has an important role in activating cell-mediated immunity following parasitic infections. It belongs to the same cytokine family as IL-3 and GM-CSF. The biologically active IL-5 consists of an antiparallel homodimer linked by disulfide bonds. Human and murine IL-5 are 70% identical at the amino acid level and show species cross-reactivity. IL-5 exerts its effects on target cells by binding to specific cell surface receptors.
PRODUCED TO ORDER
ISOkine™ recombinant human IL-5 is produced in the endosperm tissue of barley grain (Hordeum vulgare).
Sterile filtered through a 0.2 µm filter. Lyophilized from PBS, pH 7.2
Greater than 98% by SDS-PAGE gel analysis.
Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Bioactivity is assayed by measuring the dose dependent effect of human IL-5 on proliferation of TF-1 cells. The EC50 for this batch of ISOkine™ recombinant human IL-5 was determined to be 1.51 ng/ml, corresponding to 0.66x 10E6 U/mg.
Endotoxin level is less than 0.005ng per µg of ISOkine™ product (< 0.05EU/µg).
Purified ISOkine™ product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction. Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland
Recombinant human IL-5 contains 115 amino acids and a 16 a.a. histidine-based tag for a total length of 131 a.a. and has a predicted molecular mass of 15.3 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 22 and 23 kDa in SDS-PAGE.