VEGF164, mouse, recombinant
Vascular endothelial growth factor (VEGF) stimulates vascular permeability and actively mediates both angiogenesis and vasculogenesis in the fetus as well as the adult. It binds to transmembrane receptors (VEGF R1 and R2) on endothelial cells, thereby promoting endothelial proliferation, migration and survival, and inhibiting apoptosis. VEGF is required during embryogenesis and has a role in wound healing in adults. Alternately spliced isoforms of VEGF have been identified in the mouse, consisting of 120, 164 and 188 amino acids (aa). VEGF164 shares 97% aa sequence identity with the corresponding regions of rat and 89% with human, and its biological activities are not species-specific.biological activities are not species-specific.
ISOkine™ recombinant mouse VEGF164 is produced in the endosperm tissue of barley grain (Hordeum vulgare).
Sterile filtered through a 0.2 µm filter. Lyophilized from PBS, pH 7.2
Greater than 98% by SDS-PAGE gel analysis.
Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Each batch of ISOkine™ growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine™ recombinant mouse VEGF164 was determined by its dose dependent effect on proliferation of Human Umbilical Vein Endothelial Cells (HUVEC). The ED50 for this effect is typically between 1-5 ng/ml corresponding to specific activity range of 0.2 - 1 x 10e5 U/mg. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin level is less than 0.005ng per µg of ISOkine™ product (< 0.05EU/µg).
Purified ISOkine™ product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction. Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland
Recombinant mouse VEGF164 contains 164 amino acids and a 16 a.a. histidine-based tag for a total length of 180 a.a. and has a predicted molecular mass of 21.4 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 26 - 28 kDa in SDS-PAGE.