€180 per 1mg
€1.500 per 10mg
€10.000 per 100mg
IL-6 is a multifunctional cytokine that has an important role in host defence by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/ macrophages, fibroblasts and various tumour cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9kDa protein containing 185 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells
Recombinant MESOkine IL-6 is produced in the endosperm tissue of barley seed (Hordeum vulgare).
Tris pH 7.4, sterile filtered through 0.2 um filter.
MESOkine recombinant IL-6 is purified from barley seeds resulting in an extract containing purified recombinant IL-6 protein along with co-purified barley seed proteins. The amount of the recombinant IL-6 protein is typically in the range of 20-30% of total purified protein content. The exact content of the growth factor is stated on the vials. The co-purified barley seed proteins serve as stabilizing proteins that can prolong the lifetime of the recombinant protein and may enhance the bioactivity of growth factors in standardized bioassays.
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline or a solution of your choice, to a concentration of no less than 50 µg/ml. The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Each batch of MESOkine growth factor is tested for bioactivity and verified to have comparable activity to a corresponding commercial source of highly purified (>95%) growth factor. The bioactivity of MESOkine recombinant human IL-6 was determined by its dose dependent effect on proliferation of 7TD-1 cells. The ED50 for this effect using IL-6 is typically < 0.1 ng/ml corresponding to specific activity of > 10 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin levels of each MESOkine is measured and verified to have endotoxin content <0.05 EU/ug.
Recombinant human MESOkine IL-6 contains 185 amino acids and a 6 a.a. histidine tag for a total length of 191 a.a. and has a predicted molecular mass of 21.8 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 25 kDa in SDS-PAGE.