EGF

Epidermal Growth Factor (EGF) is a potent growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation. It stimulates the proliferation of various epidermal and epithelial cells. EGF has been shown to inhibit gastric secretion, promote colony formation of epidermal cells in culture, and be involved in wound healing. EGF is the prototype of a large family of EGF-like proteins, it signals through the c-erbB receptor. Recombinant human EGF is a globular protein with 3 intramolecular disulfide bonds essential for biological activity. Approximately 70% homology is found between human EGF and EGF isolated from other species. The relative positions of the cysteine residues are conserved.

Recombinant human EGF contains 53 amino acids and an N-terminal 6 a.a. histidine tag for a total length of 59 a.a. and has a predicted molecular mass of 7 kDa. The recombinant protein migrates with an apparent molecular mass of 9.5 kDa in SDS PAGE.

Produced in the endosperm tissue of barley grain (Hordeum vulgare).

The bioactivity of ISOkine® recombinant human EGF is assayed by measuring its dose-dependent effect on the proliferation of 3T3 cells.

The ED50 for this effect using EGF is typically < 0. 1 ng/ml, corresponding to a specific activity > 10 x10e6 U/mg.

We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of this ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source.

Greater than 95% by SDS-PAGE gel analysis

Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg).

Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (Mat Assay) using Human 10-plex Cytokine Assay measuring IL 6, TNF alpha, and IL 1 beta induction.

The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Highly stable as supplied for a maximum of 5 years and a minimum of 12 months from date of receipt (check expiration date specific for each lot# on Certificate of Analysis)

Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).

PBS pH 7.2, sterile filtered