EGF
€25 per 10μg
€50 per 5x10μg
€45 per 50μg
€60 per 10x10μg
€55 per 100μg
€145 per 500(5x100)μg
€165 per 10x100μg
€160 per 1000μg
€320 per 20x100μg
€315 per 2x1000μg
€310 per 2000μg
€770 per 50x100μg
€760 per 5x1000μg
€750 per 5000μg
€0 per BULK (>5mg up to gm or kg) - please inquire -g
- IK0100 10μg up to 5mg
Description
Epidermal Growth Factor (EGF) is a potent growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation. It stimulates the proliferation of various epidermal and epithelial cells. EGF has been shown to inhibit gastric secretion, promote colony formation of epidermal cells in culture, and be involved in wound healing. EGF is the prototype of a large family of EGF-like proteins, it signals through the c-erbB receptor. Recombinant human EGF is a globular protein with 3 intramolecular disulfide bonds essential for biological activity. Approximately 70% homology is found between human EGF and EGF isolated from other species. The relative positions of the cysteine residues are conserved.
Molecular Weight
Recombinant human EGF contains 53 amino acids and an N-terminal 6 a.a. histidine tag for a total length of 59 a.a. and has a predicted molecular mass of 7 kDa. The recombinant protein migrates with an apparent molecular mass of 9.5 kDa in SDS PAGE.
Source
Produced in the endosperm tissue of barley grain (Hordeum vulgare).
Biological Activity
The bioactivity of ISOkine® recombinant human EGF is assayed by measuring its dose-dependent effect on the proliferation of 3T3 cells.
The ED50 for this effect using EGF is typically < 0. 1 ng/ml, corresponding to a specific activity > 10 x10e6 U/mg.
We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of this ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source.
Purity
Greater than 95% by SDS-PAGE gel analysis
Endotoxin Level
Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg).
Pyrogen Free
Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (Mat Assay) using Human 10-plex Cytokine Assay measuring IL 6, TNF alpha, and IL 1 beta induction.
Storage
The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Stability
Highly stable as supplied for a maximum of 5 years and a minimum of 12 months from date of receipt (check expiration date specific for each lot# on Certificate of Analysis)
Reconstitution
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 µg/ml. For long-term storage of the reconstituted solution, it is recommended to add a carrier protein (0.1% HSA or BSA).
Formulation
PBS pH 7.2, sterile filtered