IL-2

IL-2, 01-A0030Human
Price

€85 per 50μg

€130 per 100μg

€430 per 1mg

€3.440 per 10mg

Catalog number

01-A0030

  • Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes, and macrophages. It is essential for proper immune response; it may also be an important factor in the natural suppression of autoimmunity. IL-2may be of use as an anti-tumor agent in cancer therapy. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity.

  • Produced in the endosperm tissue of barley grain (Hordeum vulgare).

  • PBS pH 7.2, sterile filtered through 0.2 µm filter.

  • Greater than 95% by SDS-PAGE gel analysis.

  • Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).

  • The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

  • Each batch of ISOkine growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. Bioactivity of ISOkine recombinant human IL-2 is assayed by measuring its dose dependent effect on proliferation of CTLL-2 cells. The ED50 for this effect is typically < 1.5 ng/ml, corresponding to a specific activity > 6.7 x10e5 U/mg. Optimal concentration should be determined for specific applications and cell lines.

  • Endotoxin level is less than 0.005ng per µg of ISOkine product (0.05EU/µg).

  • Purified ISOkine product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland

  • Recombinant human IL-2 contains 133 amino acids and a 16 a.a. histidine-based tag for a total length of 149 a.a. and has a predicted molecular mass of 17.5 kDa. The recombinant protein migrates with an apparent molecular mass of 22 kDa in SDS-PAGE.