IL-4

IL-4, 01-A0050Human
Price

€265 per 50μg

€410 per 100μg

€1.370 per 1mg

€10.960 per 10mg

Catalog number

01-A0050

  • IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells.

  • Produced in the endosperm tissue of barley grain (Hordeum vulgare).

  • PBS pH 7.2, sterile filtered through 0.2 µm filter.

  • Greater than 95% by SDS-PAGE gel analysis.

  • Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).

  • The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

  • Each batch of ISOkine growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine recombinant humanIL-4 was determined by its dose-dependent effects on the proliferation of TF-1 cells. The ED50 value for this effect is typically < 0.4 ng/ml, corresponding to a specific activity > 2.5 x 10e6 units/mg. Optimal concentration should be determined for specific applications and cell lines.

  • Endotoxin level is less than 0.005ng per µg of ISOkine product (0.05EU/µg).

  • Purified ISOkine product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland

  • This recombinant human FGF-basic consists of 146 amino acid residues. In addition, it contains a 10 aa tag at the Recombinant human IL-4 contains 130 amino acids and a 16 a.a. Histidine-based tag for a total length of 146 a.a. and has a predicted molecular mass of 17.2 kDa. Due to glycosylation the recombinant protein migrates as smeary band with an apparent molecular mass of 23 kDa in SDS-PAGE.