M-CSF

M-CSF, IK2400 (*01-A0220)Human
Price

€300 per 50μg

€460 per 100μg

€1.540 per 1mg

Catalog number

IK2400 (*01-A0220)

  • M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes.  It enhances antibody-dependent cell-mediated cytotoxicity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts.  Human M-CSF shows biological activity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells.

  • Produced in the endosperm tissue of barley grain (Hordeum vulgare).

  • PBS pH 7.2, sterile filtered through 0.2 µm filter.

  • Greater than 95% by SDS-PAGE gel analysis.

  • Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).

  • The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

  • Each batch of ISOkine growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine recombinant human M-CSF was determined by its dose-dependent effect on the proliferation of M-NFS-60 cells.  The ED50 value for this effect is typically < 4 ng/ml corresponding to specific activity > 2.5 x 10e5 U/mg. Optimal concentration should be determined for specific applications and cell lines.

  • Endotoxin level is less than 0.005ng per µg of ISOkine product (0.05EU/µg).

  • Purified ISOkine product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland

  • Recombinant human M-CSF contains 159 amino acids and a 16 a.a. Histidine-based tag for a total length of 175a.a. and has a predicted molecular mass of 20.7 kDa.  The recombinant protein migrates with an apparent molecular mass of 24-26 kDa in SDS-PAGE.