Transferrin

Transferrin, MK1601Bovine
Price
Catalog number

MK1601

  • Transferrin is a major mammalian serum glycoprotein, transporting iron from sites of absorption and storage to tissue cells. Transferrin contains two homologous iron-binding domains, each of which binds one Fe3+. Transferrin transports iron in the bloodstream and delivers it to various tissues, after binding to a specific receptor (TfR) on the cell surface. Once inside, the iron is released into the cells and the iron-free transferrin also referred to as apotransferrin, is exocytosed outside the cell without being degraded. Transferrin is known to deliver iron to cells in a physiologically safe and effective form.

  • Recombinant MESOkine® bovine Transferrin, is produced in the endosperm tissue of barley seed (Hordeum vulgare).

  • Tris pH 7.2, sterile filtered through 0.2 um filter.

  • MESOkine® recombinant bovine Transferrin is purified from barley seeds resulting in an extract containing purified recombinant Transferrin along with co-purified barley seed proteins. The exact content of the recombinant protein is stated on the vials. The co-purified barley seed proteins may serve as stabilizing proteins that can prolong the lifetime of the recombinant protein during proper storage.

  • It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline or a solution of your choice, preferably to a concentration of no more than 10-100 μg/ml. If needed the reconstituted material may be centrifuged at 3500G for 5 min or filtrated using a 0.22 micron filter. The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

  • Has not been tested. More information on specific lots can be found on the Transferrin information sheet included in the shipment.

  • MESOkine® bovine Transferrin contains 685 amino acids and an N-terminal 6 a.a. histidine tag for a total length of 691 a.a. and has a predicted molecular mass of 76.7 kDa. The recombinant protein migrates with an apparent molecular mass of approximately 80 kDa in SDS-PAGE.