Transferrin is a major mammalian serum glycoprotein, transporting iron from sites of absorption and storage to tissue cells. Transferrin contains two homologous iron-binding domains, each of which binds one Fe3+. Transferrin transports iron in the bloodstream and delivers it to various tissues, after binding to a specific receptor (TfR) on the cell surface. Once inside, the iron is released into the cells and the iron-free transferrin also referred to as apotransferrin, is exocytosed outside the cell without being degraded. Transferrin is known to deliver iron to cells in a physiologically safe and effective form.
Recombinant MESOkine® bovine Transferrin, is produced in the endosperm tissue of barley seed (Hordeum vulgare).
Tris pH 7.2, sterile filtered through 0.2 um filter.
MESOkine® recombinant bovine Transferrin is purified from barley seeds resulting in an extract containing purified recombinant Transferrin along with co-purified barley seed proteins. The amount of the recombinant Transferrin is typically in the range of 20-30% of total purified protein content. The exact content of the recombinant protein is stated on the vials. The co-purified barley seed proteins may serve as stabilizing proteins that can prolong the lifetime of the recombinant protein during proper storage.
It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline or a solution of your choice, preferably to a concentration of no more than 10-100 μg/ml. If needed the reconstituted material may be centrifuged at 3500G for 5 min or filtrated using a 0.22 micron filter. The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Has not been tested. More information on specific lots can be found on the Transferrin information sheet included in the shipment.
MESOkine® bovine Transferrin contains 685 amino acids and an N-terminal 6 a.a. histidine tag for a total length of 691 a.a. and has a predicted molecular mass of 76.7 kDa. The recombinant protein migrates with an apparent molecular mass of approximately 80 kDa in SDS-PAGE.