FGF basic

FGF-basic (bFGF or FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members are heparin-binding multifunctional proteins and are involved in a variety of biological processes including angiogenesis, wound healing, and embryonic development. FGF-basic is known to play a key role during prenatal development and postnatal growth and regeneration of tissues by promoting cellular proliferation and differentiation. FGF basic has been recognized as a critical component of human embryonic stem cell culture medium due to its vital role for cells to remain in an undifferentiated state. FGF basic signals through FGFR 1b, 1c, 2c, 3c and 4. This recombinant human FGF-basic consists of 146 amino acid residues. In addition it contains a 10 aa tag at the N terminus resulting in a 17.8 kDa protein.

Produced in the endosperm tissue of barley grain (Hordeum vulgare).

PBS pH 7.2, sterile filtered

Greater than 95% by SDS-PAGE gel analysis.

Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile phosphate buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).

The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze thaw cycles.

Each batch of ISOkine growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. Bioactivity of ISOkine recombinant human FGF-basic 146 is assayed by measuring its dose dependent effect on proliferation of 3T3 cells. The ED50 for this effect using FGFb146 is typically < 0.4 ng/ml, corresponding to a specific activity > 2.5 x10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.

Endotoxin level is less than 0.005ng per µg of ISOkine product (0.05EU/µg).

Purified ISOkine product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL 6, TNF alpha and IL 1beta induction. ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland

This recombinant human FGF-basic consists of 146 amino acid residues. In addition it contains a 10 aa tag at the N-terminus resulting in a 17.8 kDa protein.