IL-6

IL-6 is a multifunctional cytokine with an essential role in host defense by regulating immune and inflammatory responses. It is expressed by various cell types, including T cells, B cells, monocytes/macrophages, fibroblasts, and possibly tumor cells. IL-6 has diverse biological functions, stimulating B-cell differentiation and antibody production, synergizing with other factors in hematopoiesis and platelet production, inducing hepatic acute-phase protein expression, and regulating bone metabolism. Recombinant bovine IL-6 has a molecular weight of 21.5kDa containing 185 amino acid residues.

Produced in the endosperm tissue of barley grain (Hordeum vulgare).

PBS pH 7.2, sterile filtered through 0.2 µm filter

Greater than 95% by SDS-PAGE gel analysis.

Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the recon statute solution it is recommended to add a carrier protein (0.1% HSA or BSA).

The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Each batch of ISOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.

Endotoxin level is less than 0.005ng per µg of ISOkine® product (0.05EU/µg).

Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland