IL-6, bovine

IL-6 is a multifunctional cytokine with an essential role in host defense by regulating immune and inflammatory responses. It is expressed by various cell types, including T cells, B cells, monocytes/macrophages, fibroblasts, and possibly tumor cells. IL-6 has diverse biological functions, stimulating B-cell differentiation and antibody production, synergizing with other factors in hematopoiesis and platelet production, inducing hepatic acute-phase protein expression, and regulating bone metabolism. Recombinant bovine IL-6 has a molecular weight of 21.5kDa containing 185 amino acid residues.

Recombinant MESOkine® IL-6, bovine is produced in the endosperm tissue of barley seed (Hordeum vulgare).

2xPBS pH 7.2, sterile filtered through 0.2 um filter.

MESOkine® recombinant IL-6 is purified from barley seeds resulting in an extract containing purified recombinant IL-6 protein along with co-purified barley seed proteins. The exact content of the growth factor is stated on the vials. The co-purified barley seed proteins may serve as stabilizing proteins that can prolong the lifetime of the recombinant protein during proper storage and may enhance the bioactivity of growth factors in cell culture.

It is recommended to reconstitute the lyophilized protein in sterile buffered saline or a solution of your choice, preferably to a concentration of no more than 10-100 µg/ml. If needed the reconstituted material may be centrifuged at 3500G for 5 min or filtrated using 0.45 micron filter. The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Each batch of MESOkine® growth factor is tested for bioactivity and verified to have comparable activity to a corresponding commercial source of highly purified (>95%) growth factor. The bioactivity of MESOkine® recombinant human IL-6 was determined by its dose dependent effect on proliferation of 7TD-1 cells. The ED50 for this effect using IL-6 is typically < 0.1 ng/ml corresponding to specific activity of > 10 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.

Recombinant human MESOkine® IL-6 contains 185 amino acids and a 6 a.a. histidine tag for a total length of 191 a.a. and has a predicted molecular mass of 21.8 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 25 kDa in SDS-PAGE.