ISOkine® Premium · IK1700
Flt-3 ligand (human)
Recombinant Flt-3 ligand (human) for early hematopoiesis, dc expansion. Animal-free and endotoxin-free, produced in barley with >95% purity and <0.01 EU/µg endotoxin, and batch-to-batch consistency you can validate once and scale.
Datasheets, SDS, and Certificate of Origin available for download or on request.
About Flt-3 ligand (human)
Flt-3 ligand (FMS related tyrosine kinase 3 ligand, flk-2 ligand) is involved in proliferation and differentiation of early hematopoietic cells. Flt-3 ligand synergizes with other CSF’s and interleukins to induce proliferation of early hematopoietic cells but does not stimulate growth or differentiation alone. Flt-3 ligand binds to cells expressing the tyrosine kinase receptor Flt3. Multiple isoforms of Flt-3 ligand have been identified but the predominant biologically active isoform is a transmembrane protein which can be proteolytically cleaved to generate a biologically active soluble form.
Biological activity
The bioactivity of ISOkine® recombinant human Flt-3 ligand was assayed by measuring its dose dependent effect on proliferation of AML5 cells.
The ED50 value for this ISOkine is < 0.3 ng/ml corresponding to specific activity > 3.3 x 10e6 U/mg.
We recommend that the optimal concentration for each specific application be determined by an initial dose response assay. Each batch of this ISOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.
At a glance
Specifications
Starting price for research quantities. Volume pricing on request.
Purity verified by SDS-PAGE gel analysis on every lot.
Produced in an endotoxin-free barley host, tested per batch.
Datasheet specifications
| Property | Detail |
|---|---|
| Source | Produced in the endosperm tissue of barley grain (Hordeum vulgare). |
| Molecular Weight | Recombinant human Flt-3 ligand contains 156 amino acids and a 16 a.a. Histidine-based tag for a total length of172 a.a. and has a predicted molecular mass of 19.9 kDa. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 20 and 22 kDa in SDS-PAGE. |
| Purity | Greater than 95% by SDS-PAGE gel analysis. |
| Endotoxin Level | Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg). |
| Pyrogen Free | Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction. |
| Storage | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Stability | Highly stable as supplied for a maximum of 5 years and a minimum of 12 months from date of receipt (check expiration date specific for each lot# on Certificate of Analysis) |
| Reconstitution | Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Formulation | PBS pH 7.2, sterile filtered through 0.2 µm filter. |
Size and pricing
| Pack size | List price (EUR) |
|---|---|
| 10 µg | €125 |
| 5 × 10 µg | €350 |
| 50 µg | €325 |
| 10 × 10 µg | €525 |
| 100 µg | €500 |
| 5 × 100 µg | €1700 |
| 10 × 100 µg | €2750 |
| 1000 µg | €2650 |
| 20 × 100 µg | €4900 |
| 2 × 1000 µg | €4500 |
| 2000 µg | €4000 |
| 50 × 100 µg | €9900 |
| 5 × 1000 µg | €9500 |
| 5000 µg | €8990 |
| Bulk (> 5 mg) | Please inquire |