ISOkine® Premium · IK1800
G-CSF (human)
Recombinant G-CSF (human) for hematopoiesis, neutrophil expansion. Animal-free and endotoxin-free, produced in barley with >95% purity and <0.01 EU/µg endotoxin, and batch-to-batch consistency you can validate once and scale.
Datasheets, SDS, and Certificate of Origin available for download or on request.
About G-CSF (human)
Granulocyte Colony-Stimulating Factor (G-CSF, filgrastim, pegfilgrastim or lenograstim) is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage, in a dose-dependent manner. It is produced by a variety of cells including macrophages, monocytes, fibroblasts, endothelial cells, and bone marrow stroma in response to specific stimulation by f.ex. endotoxin, TNF-alpha and IFN-gamma. Clinical applications of G-CSF include treatment of leukopenia following cancer therapy and bone marrow transplantation. Human and murine G-CSF are cross-species reactive.
Biological activity
The bioactivity of ISOkine® recombinant human G-CSF was assayed by measuring its dose dependent effect on proliferation of M-NFS-60 cells.
The ED50 value for this ISOkine® is < 0.1 ng/ml corresponding to specific activity > 1 x 10e7 U/mg.
We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.
G-CSF (human) bioactivity: dose-dependent response assay.
At a glance
Specifications
Starting price for research quantities. Volume pricing on request.
Purity verified by SDS-PAGE gel analysis on every lot.
Produced in an endotoxin-free barley host, tested per batch.
Datasheet specifications
| Property | Detail |
|---|---|
| Source | Produced in the endosperm tissue of barley grain (Hordeum vulgare). |
| Molecular Weight | Recombinant human G-CSF contains a 18.7 kDa protein with 174 amino acids and a 16 a.a. Histidine-based tag for a total length of 190a.a. and has a predicted molecular mass of 20.8 kDa. |
| Purity | Greater than 95% by SDS-PAGE gel analysis. |
| Endotoxin Level | Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg). |
| Pyrogen Free | Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. |
| Storage | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Stability | Highly stable as supplied for a maximum of 5 years and a minimum of 12 months from date of receipt (check expiration date specific for each lot# on Certificate of Analysis) |
| Reconstitution | Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Formulation | PBS pH 7.2, sterile filtered through 0.2 µm filter. |
Size and pricing
| Pack size | List price (EUR) |
|---|---|
| 10 µg | €125 |
| 5 × 10 µg | €350 |
| 50 µg | €325 |
| 10 × 10 µg | €525 |
| 100 µg | €500 |
| 5 × 100 µg | €1700 |
| 10 × 100 µg | €2750 |
| 1000 µg | €2650 |
| 20 × 100 µg | €4999 |
| 2 × 1000 µg | €4750 |
| 2000 µg | €4500 |
| 50 × 100 µg | €9900 |
| 5 × 1000 µg | €9500 |
| 5000 µg | €8990 |
| Bulk (> 5 mg) | Please inquire |