ISOkine® Premium · IK2100
IL-2 (human)
Recombinant IL-2 (human) for t-cell expansion, immune cell culture. Animal-free and endotoxin-free, produced in barley with >95% purity and <0.01 EU/µg endotoxin, and batch-to-batch consistency you can validate once and scale.
Datasheets, SDS, and Certificate of Origin available for download or on request.
About IL-2 (human)
Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes, and macrophages. It is essential for proper immune response; it may also be an important factor in the natural suppression of autoimmunity. IL-2may be of use as an anti-tumor agent in cancer therapy. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity.
Biological activity
The bioactivity of ISOkine® recombinant human IL-2 is assayed by measuring its dose dependent effect on proliferation of CTLL-2 cells.
The ED50 for this effect is < 1.5 ng/ml, corresponding to a specific activity > 6.7 x10e5 U/mg.
We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.
IL-2 (human) bioactivity: dose-dependent response assay.
At a glance
Specifications
Starting price for research quantities. Volume pricing on request.
Purity verified by SDS-PAGE gel analysis on every lot.
Produced in an endotoxin-free barley host, tested per batch.
Datasheet specifications
| Property | Detail |
|---|---|
| Source | Produced in the endosperm tissue of barley grain (Hordeum vulgare). |
| Molecular Weight | Recombinant human IL-2 contains 133 amino acids and a 16 a.a. histidine-based tag for a total length of 149 a.a. and has a predicted molecular mass of 17.5 kDa. The recombinant protein migrates with an apparent molecular mass of 22 kDa in SDS-PAGE. |
| Purity | Greater than 95% by SDS-PAGE gel analysis. |
| Endotoxin Level | Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg). |
| Pyrogen Free | Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. |
| Storage | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Stability | Highly stable as supplied for a maximum of 5 years and a minimum of 12 months from date of receipt (check expiration date specific for each lot# on Certificate of Analysis). |
| Reconstitution | Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Formulation | PBS pH 7.2, sterile filtered through 0.2 µm filter. |
Size and pricing
| Pack size | List price (EUR) |
|---|---|
| 10 µg | €60 |
| 5 × 10 µg | €100 |
| 50 µg | €90 |
| 10 × 10 µg | €140 |
| 100 µg | €130 |
| 5 × 100 µg | €350 |
| 10 × 100 µg | €550 |
| 1000 µg | €525 |
| 20 × 100 µg | €1000 |
| 2 × 1000 µg | €950 |
| 2000 µg | €900 |
| 50 × 100 µg | €2250 |
| 5 × 1000 µg | €2125 |
| 5000 µg | €1990 |
| Bulk (> 5 mg) | Please inquire |