ISOkine® Premium · IK2300
IL-4 (human)
Recombinant IL-4 (human) for th2 differentiation, b-cell. Animal-free and endotoxin-free, produced in barley with >95% purity and <0.01 EU/µg endotoxin, and batch-to-batch consistency you can validate once and scale.
Datasheets, SDS, and Certificate of Origin available for download or on request.
About IL-4 (human)
IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells.
Biological activity
The bioactivity of ISOkine® recombinant human IL-4 was assayed by measuring its dose dependent effect on proliferation of TF-1 cells.
The ED50 value for this effect is < 0.4 ng/ml, corresponding to a specific activity > 2.5 x 10e6 U/mg.
We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of this ISOkine® growth factors is tested for bioactivity and verified to have comparable activity to a commercial source.
At a glance
Specifications
Starting price for research quantities. Volume pricing on request.
Purity verified by SDS-PAGE gel analysis on every lot.
Produced in an endotoxin-free barley host, tested per batch.
Datasheet specifications
| Property | Detail |
|---|---|
| Source | Produced in the endosperm tissue of barley grain (Hordeum vulgare). |
| Molecular Weight | This recombinant human IL-4 consists of 146 amino acid residues. In addition, it contains a 10 aa tag at the Recombinant human IL-4 contains 130 amino acids and a 16 a.a. Histidine-based tag for a total length of 146 a.a. and has a predicted molecular mass of 17.2 kDa. Due to glycosylation, the recombinant protein migrates as a smeary band with an apparent molecular mass of 23 kDa in SDS-PAGE. |
| Purity | Greater than 95% by SDS-PAGE gel analysis. |
| Endotoxin Level | Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg). |
| Pyrogen Free | Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. |
| Storage | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Stability | Highly stable as supplied for a maximum of 5 years and a minimum of 12 months from date of receipt (check expiration date specific for each lot# on Certificate of Analysis) |
| Reconstitution | Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Formulation | PBS pH 7.2, sterile filtered through 0.2 µm filter. |
Size and pricing
| Pack size | List price (EUR) |
|---|---|
| 10 µg | €65 |
| 5 × 10 µg | €275 |
| 50 µg | €260 |
| 10 × 10 µg | €425 |
| 100 µg | €400 |
| 5 × 100 µg | €1250 |
| 10 × 100 µg | €2100 |
| 1000 µg | €2000 |
| 20 × 100 µg | €3750 |
| 2 × 1000 µg | €3600 |
| 2000 µg | €3500 |
| 50 × 100 µg | €8500 |
| 5 × 1000 µg | €8250 |
| 5000 µg | €7990 |
| Bulk (> 5 mg) | Please inquire |