ISOkine® Premium · IK0700
LIF (human)
Recombinant LIF (human) for esc maintenance, hematopoiesis. Animal-free and endotoxin-free, produced in barley with >95% purity and <0.01 EU/µg endotoxin, and batch-to-batch consistency you can validate once and scale.
Datasheets, SDS, and Certificate of Origin available for download or on request.
About LIF (human)
Leukemia inhibitory factor (LIF) is a pleiotropic lymphoid factor belonging to the interleukin-6 family of cytokines. It was initially described as a factor inhibiting the proliferation of myeloid leukemia cells and inducing their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis and roles in cholinergic neuron differentiation, hematopoeisis, inflammation and bone metabolism. LIF inhibits the differentiation of embryonic stem cells, it is therefore important in ES cell culture. Human and mouse LIF share 78% amino acid homology, the human version is equally active on human and mouse cells but murine LIF is not active on human cells.
Biological activity
The bioactivity of ISOkine human LIF was determined by its ability to induce proliferation of TF-1 cells in a dose dependent manner.
The ED50 value for this effect is <0.3 ng/ml corresponding to specific activity of >3.3 x 10e6 U/mg.
We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.
LIF (human) bioactivity: dose-dependent response assay.
At a glance
Specifications
Starting price for research quantities. Volume pricing on request.
Purity verified by SDS-PAGE gel analysis on every lot.
Produced in an endotoxin-free barley host, tested per batch.
Datasheet specifications
| Property | Detail |
|---|---|
| Source | Produced in the endosperm tissue of barley grain (Hordeum vulgare). |
| Molecular Weight | Recombinant human LIF contains 180 amino acids and a 16 a.a. histidine-based tag for a total length of 196 a.a. and has a predicted molecular mass of 21.8 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 25-30 kDa in SDS-PAGE. |
| Purity | Greater than 90-98% by SDS-PAGE gel analysis. |
| Endotoxin Level | Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg). |
| Pyrogen Free | Purified ISOkine product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using (MAT Assay)Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. |
| Storage | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Stability | Highly stable as supplied for a maximum of 5 years and a minimum of 12 months from date of receipt (check expiration date specific for each lot# on Certificate of Analysis) |
| Reconstitution | Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Formulation | PBS pH 7.2, sterile filtered through 0.2 µm filter. |
Size and pricing
| Pack size | List price (EUR) |
|---|---|
| 10 µg | €75 |
| 5 × 10 µg | €275 |
| 50 µg | €250 |
| 10 × 10 µg | €400 |
| 100 µg | €375 |
| 5 × 100 µg | €1250 |
| 10 × 100 µg | €2100 |
| 1000 µg | €2000 |
| 20 × 100 µg | €3750 |
| 2 × 1000 µg | €3600 |
| 2000 µg | €3500 |
| 50 × 100 µg | €8500 |
| 5 × 1000 µg | €8250 |
| 5000 µg | €7990 |
| Bulk (> 5 mg) | Please inquire |