ISOkine® Premium · IK2400
M-CSF (human)
Recombinant M-CSF (human) for monocyte/macrophage differentiation. Animal-free and endotoxin-free, produced in barley with >95% purity and <0.01 EU/µg endotoxin, and batch-to-batch consistency you can validate once and scale.
Datasheets, SDS, and Certificate of Origin available for download or on request.
About M-CSF (human)
M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxicity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological activity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells.
Biological activity
The bioactivity of ISOkine® recombinant human M-CSF was assayed by measuring its dose dependent effect on proliferation of M-NFS-60 cells.
The ED50 value for this effect is < 4 ng/ml corresponding to specific activity > 2.5 x 10e5 U/mg.
We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.
[Bioassay graphic](https://images.prismic.io/orfgenetics/abPxs1xvIZEnjpYa_M-CSF.png?auto=format,compress?auto=compress,format)
At a glance
Specifications
Starting price for research quantities. Volume pricing on request.
Purity verified by SDS-PAGE gel analysis on every lot.
Produced in an endotoxin-free barley host, tested per batch.
Datasheet specifications
| Property | Detail |
|---|---|
| Source | Produced in the endosperm tissue of barley grain (Hordeum vulgare). |
| Molecular Weight | Recombinant human M-CSF contains 159 amino acids and a 16 a.a. Histidine-based tag for a total length of 175a.a. and has a predicted molecular mass of 20.7 kDa. The recombinant protein migrates with an apparent molecular mass of 24-26 kDa in SDS-PAGE. |
| Purity | Greater than 95% by SDS-PAGE gel analysis. |
| Endotoxin Level | Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg). |
| Pyrogen Free | Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. |
| Storage | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Stability | Highly stable as supplied for a maximum of 5 years and a minimum of 12 months from date of receipt (check expiration date specific for each lot# on Certificate of Analysis). |
| Reconstitution | Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Formulation | PBS pH 7.2, sterile filtered through 0.2 µm filter. |
Size and pricing
| Pack size | List price (EUR) |
|---|---|
| 10 µg | €125 |
| 5 × 10 µg | €350 |
| 50 µg | €325 |
| 10 × 10 µg | €525 |
| 100 µg | €500 |
| 5 × 100 µg | €1700 |
| 10 × 100 µg | €2750 |
| 1000 µg | €2650 |
| 20 × 100 µg | €4999 |
| 2 × 1000 µg | €4750 |
| 2000 µg | €4500 |
| 50 × 100 µg | €9900 |
| 5 × 1000 µg | €9500 |
| 5000 µg | €8990 |
| Bulk (> 5 mg) | Please inquire |