ISOkine® Premium · IK2600
TNF-alpha (human)
Recombinant TNF-alpha (human) for inflammation, apoptosis, t-cell. Animal-free and endotoxin-free, produced in barley with >95% purity and <0.01 EU/µg endotoxin, and batch-to-batch consistency you can validate once and scale.
Datasheets, SDS, and Certificate of Origin available for download or on request.
About TNF-alpha (human)
TNFα (Tumor necrosis factor, alpha), a member of the TNF ligand superfamily, is among others, secreted by macrophages, activated monocytes, neutrophils, T-cells, B-cells and natural killer cells stimulated by bacterial lipopolysaccharides. TNF-alpha shows approximately 30% homology with TNF-beta. TNF-alpha plays an important role in the regulation of immune cells. TNFα has many biological activities, including a cytotoxic effect to various tumor cells, causes inflammation, cellular proliferation and plays a critical role in mediating resistance to bacterial infections. Human and murine TNF-alpha show approximately 79% homolgy and human TNF-alpha is active on murine cells with a slightly reduced specific activity
Biological activity
The bioactivity of ISOkine® recombinant human TNF-alpha is assayed by measuring its dose dependent effect in a cytotoxicity assay using Actinomycin-D sensitized L929 cells.
The ED50 for this effect is < 0.4 ng/ml corresponding to a specific activity > 2.5 x10e6 U/mg.
We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.
[Bioactivity graphic](https://images.prismic.io/orfgenetics/abABtVxvIZEnjhnc_TNF-alpha.png?auto=format,compress?auto=compress,format)
At a glance
Specifications
Starting price for research quantities. Volume pricing on request.
Purity verified by SDS-PAGE gel analysis on every lot.
Produced in an endotoxin-free barley host, tested per batch.
Datasheet specifications
| Property | Detail |
|---|---|
| Source | Produced in the endosperm tissue of barley grain (Hordeum vulgare). |
| Molecular Weight | Recombinant human TNF-alpha is a 19.6 kDa protein containing 158 amino acid residues and contains a 16 a.a. Histidine-based tag for a total length of 174 a.a. The recombinant protein migrates an apparent molecular mass of 24 kDa in SDS-PAGE. |
| Purity | Greater than 95% by SDS-PAGE gel analysis. |
| Endotoxin Level | Endotoxin level is < 0.01 EU per μg of ISOkine® product (< 0.01 EU/μg). |
| Pyrogen Free | Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. |
| Storage | The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Stability | Highly stable as supplied for a maximum of 5 years and a minimum of 12 months from date of receipt (check expiration date specific for each lot# on Certificate of Analysis). |
| Reconstitution | Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Formulation | PBS pH 7.2, sterile filtered through 0.2 µm filter. |
Size and pricing
| Pack size | List price (EUR) |
|---|---|
| 10 µg | €50 |
| 5 × 10 µg | €140 |
| 50 µg | €130 |
| 10 × 10 µg | €225 |
| 100 µg | €200 |
| 5 × 100 µg | €650 |
| 10 × 100 µg | €925 |
| 1000 µg | €900 |
| 20 × 100 µg | €1600 |
| 2 × 1000 µg | €1550 |
| 2000 µg | €1500 |
| 50 × 100 µg | €3600 |
| 5 × 1000 µg | €3550 |
| 5000 µg | €3500 |
| Bulk (> 5 mg) | Please inquire |