ISOkine® Premium (IK3200)
IL-7, human
Recombinant IL-7, human for T/B-cell development, expansion, survival. Animal-free and endotoxin-free, produced in barley with ≥95% purity and <0.01 EU/µg endotoxin, and batch-to-batch consistency you can validate once and scale.
Datasheets, SDS, and Certificate of Origin available for download or on request.
About IL-7, human
Interleukin-7 is a hematopoietic cytokine that plays an essential role in the development, expansion, and survival of T-cells (naive and memory) and B-cells. It helps regulate the number of mature lymphocytes and maintain lymphoid homeostasis by interaction with receptors composed of IL7RA and gamma/CSF2RG, activating various kinases including JAK1 and JAK3.
Biological activity
The bioactivity of ISOkine® recombinant human IL-7 is assayed by measuring its dose-dependent effect on the proliferation of 2E8 cells.
The ED50 for this effect using IL-7 is typically ≤2 ng/ml.
We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.
ISOkine® Premium, supplied as a lyophilized vial. Product-specific bioactivity data available on request.
Datasheet specifications
| Property | Detail |
|---|---|
| Source | Produced in the endosperm tissue of barley grain (Hordeum vulgare). |
| Molecular Weight | Recombinant human IL-7 contains 152 amino acids and an N-terminal 17 amino acid histidine tag for a total length of 169 amino acids and has a predicted molecular mass of 19.7 kDa. |
| Purity | ≥95% by SDS-PAGE gel analysis. |
| Endotoxin Level | Endotoxin level is <0.0100 EU per µg of ISOkine® product (<0.01 EU/µg) by LAL method. |
| Pyrogen Free (MAT Assay) | Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. |
| Storage | The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Shelf Life | 3 years when stored at < -20°C. |
| Reconstitution | Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Formulation | 2x PBS pH 7.2, sterile filtered through 0.22 µm filter. |