ISOkine® Premium (IK1100)
NRG-1, human
Recombinant NRG-1, human for growth, survival, proliferation, differentiation. Animal-free and endotoxin-free, produced in barley with ≥95% purity and <0.05 EU/µg endotoxin, and batch-to-batch consistency you can validate once and scale.
Datasheets, SDS, and Certificate of Origin available for download or on request.
About NRG-1, human
Neuregulin-1 (NRG-1) promotes cell growth, survival, proliferation and differentiation. NRG-1 is a signaling protein of the EGF family that acts primarily as a direct ligand for the ErbB3 (HER3) and ErbB4 (HER4) receptor tyrosine kinases, which in turn partners with ErbB2 (HER2) to activate downstream PI3K/AKT and MAPK signaling.
Biological activity
The bioactivity of ISOkine® recombinant human NRG-1 is assayed by measuring its dose-dependent effect on the proliferation of MCF-7 cells.
The ED50 for this effect using NRG-1 is typically ≤2 ng/ml.
We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.
ISOkine® Premium, supplied as a lyophilized vial. Product-specific bioactivity data available on request.
Datasheet specifications
| Property | Detail |
|---|---|
| Source | Produced in the endosperm tissue of barley grain (Hordeum vulgare). |
| Molecular Weight | Recombinant human NRG-1 contains 68 amino acids and an N-terminal 10 amino acid histidine tag for a total length of 78 amino acids and has a predicted molecular mass of 9.3 kDa. |
| Purity | ≥95% by SDS-PAGE gel analysis. |
| Endotoxin Level | Endotoxin level is <0.0500 EU per µg of ISOkine® product (<0.05 EU/µg) by LAL method. |
| Pyrogen Free (MAT Assay) | Purified ISOkine® product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction. |
| Storage | The lyophilized protein, though stable at room temperature for a few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Shelf Life | 3 years when stored at < -20°C. |
| Reconstitution | Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Formulation | 2x PBS pH 7.2, sterile filtered through 0.22 µm filter. |